Optimization of exonuclease digestion for isolation of DNA adducts

Title:
Optimization of exonuclease digestion for isolation of DNA adducts
Creator:
Calhoun, Shawna (Author)
Language:
English
Publisher:
May 01, 2007
Date created:
May 01, 2007
Type of resource:
Text
Genre:
Other
Format:
electronic
Digital origin:
born digital
Abstract/Description:

With many researchers concentrating on a cure for cancer, there are some focusing on detecting genetic predispositions early enough for preventative measures. The Environmental Cancer Research Program (ERCP) is one such place. Specific research is being done on DNA adducts, however the bigger bulkier adducts are having low yields. This research focused on optimizing the conditions of the enzyme used to detach the DNA adducts from DNA. The data showed that that extended incubation of the enzyme at 45°C does not diminish the enzymatic rate, and increasing the concentration of Mg2+ in the buffer results in a lower enzymatic rate after extended incubation at 55°C.

Subjects and keywords:
Cancer, Genetics
DNA adducts
enzyme rate
phosphodiesterase I

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