Toxicity of CdSe nanoparticles in Caco-2 cell cultures

Title:
Toxicity of CdSe nanoparticles in Caco-2 cell cultures
Creator:
Wang, Lin (Author)
Nagesha, Dattatri K. (Author)
Selvarasah, Selvapraba (Author)
Dokmeci, Mehmet R. (Author)
Carrier, Rebecca L. (Author)
Language:
English
Publisher:
BioMedCentral
Type of resource:
Text
Genre:
Articles
Format:
electronic
Digital origin:
born digital
Abstract/Description:
Background
Potential routes of nanomaterial exposure include inhalation, dermal contact, and ingestion. Toxicology of inhalation of ultra-fine particles has been extensively studied; however, risks of nanomaterial exposure via ingestion are currently almost unknown. Using enterocyte-like Caco-2 cells as a small intestine epithelial model, the possible toxicity of CdSe quantum dot (QD) exposure via ingestion was investigated. Effect of simulated gastric fluid treatment on CdSe QD cytotoxicity was also studied.

Results
Commercially available CdSe QDs, which have a ZnS shell and poly-ethylene glycol (PEG) coating, and in-house prepared surfactant coated CdSe QDs were dosed to Caco-2 cells. Cell viability and attachment were studied after 24 hours of incubation. It was found that cytotoxicity of CdSe QDs was modulated by surface coating, as PEG coated CdSe QDs had less of an effect on Caco-2 cell viability and attachment. Acid treatment increased the toxicity of PEG coated QDs, most likely due to damage or removal of the surface coating and exposure of CdSe core material. Incubation with un-dialyzed in-house prepared CdSe QD preparations, which contained an excess amount of free Cd2+, resulted in dramatically reduced cell viability.

Conclusion
Exposure to CdSe QDs resulted in cultured intestinal cell detachment and death; cytotoxicity depended largely, however, on the QD coating and treatment (e.g. acid treatment, dialysis). Experimental results generally indicated that Caco-2 cell viability correlated with concentration of free Cd2+ ions present in cell culture medium. Exposure to low (gastric) pH affected cytotoxicity of CdSe QDs, indicating that route of exposure may be an important factor in QD cytotoxicity.
Comments:
Originally published in Journal of Nanobiotechnology, v.6 no.11 (2008). doi:10.1186/1477-3155-6-11
Subjects and keywords:
Nanostructured materials - Toxicology
nanomaterial exposure
toxicology
Caco-2 cells
ingestion
Nanoscience and Nanotechnology
Toxicology

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