Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles

Title:
Near infrared planar tumor imaging and quantification using nanosized Alexa 750-labeled phospholipid micelles
Creator:
Papagiannaros, Aristarchos (Author)
Kale, Amit (Author)
Levchenko, Tatyana S. (Author)
Mongayt, Dmitry (Author)
Hartner, William C. (Author)
Torchilin, V. P. (Author)
Language:
English
Publisher:
Dovepress
Copyright date:
2009
Type of resource:
Text
Genre:
Articles
Format:
electronic
Digital origin:
born digital
Abstract/Description:
A novel highly biocompatible near infrared nanosized contrast agent was developed and used for rapid tumor detection and quantification using planar optical imaging and analysis. With this in mind, the near infrared fluorescent dye Alexa 750 was covalently attached to polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugate, and double labeled (with Alexa and rhodamine) PEG-PE micelles were injected into mice and observed using planar optical imaging. Pixel intensity data from the tumor site were normalized versus the autofluorescence of the animal at the same time point and normalized as signal to noise over the scattered light from the various tissues of the mice. The detected signal from the tumor was higher than the background noise allowing for rapid detection of the tumor. The tumor was clearly visible within one hour. Some signal was also detected from the abdomen of the mice. As determined by microscopy analysis, other organs of accumulation were the liver and kidney, which corresponded well to the data from the whole body imaging animal studies.
Comments:
Published in the International Journal of Nanomedicine, 2009:4, 123-131
Subjects and keywords:
Tumors - Photgraphic measurement
Infrared imaging
Micelles
nanotechnology
nanomedicine
cancer imaging agents
near infrared imaging
micelles
Pharmacy and Pharmaceutical Sciences
Permanent Link:
http://hdl.handle.net/2047/d20000805

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