A comparative study of optical fluorescent nanosensors (“PEBBLEs”) and fiber optic microsensors for oxygen sensing

Title:
A comparative study of optical fluorescent nanosensors ("PEBBLEs") and fiber optic microsensors for oxygen sensing
Creator:
Chen-Esterlit, Z. (Author)
Peteu, S. F. (Author)
Clark, Heather (Author)
McDonald, W. (Author)
Kopelman, R. (Author)
Language:
English
Publisher:
SPIE
Copyright date:
1999
Type of resource:
Text
Genre:
Articles
Format:
electronic
Digital origin:
born digital
Abstract/Description:
In this paper we report the use of phase sensitive fluorometry to obtain preliminary results from opto-chemical fluorescent oxygen nanosensors. PEBBLE (Probe Encapsulated By Biologically Localized Embedding) sensors were fabricated by immobilizing tris(4,7-diphenyl—1, 10-phenanthroline)Ru(II) chloride and tris(1,10-phenanthroline)Ru(II) chloride within a polyacrylamide matrix. PEBBLEs have diameters of 20-200 nm and exhibit excellent performance for dissolved oxygen detection. Their performance is compared with micrometer-sized (10-20 μm) optical fiber sensors and free dye in solution. Oxygen sensing ability of PEBBLEs was tested in the presence of other quenchers and compared with free dyes in solution. While PEBBLEs have been developed for minimally invasive intracellular chemical analysis, they show additional advantages, such as increased dynamic range, compared to microsensors, and an absence of interference (quenching) by heavy ions, in contrast to free dye solutions.
Comments:
Originally published in SPIE Conference on Advances in Fluorescence Technology IV, San Jose, CA, Jan. 1999, SPIE Vol. 3602, Proc. SPIE 3602, 156 (1999). DOI:10.1117/12.347517
Subjects and keywords:
Fluorimetry
Optical detectors
Oxygen - Physiological transport
nanosensor
microsensor
optode
oxygen sensor
phase modulation
Bioimaging and biomedical optics
Nanoscience and Nanotechnology

Downloads