Optochemical nanosensors for intracellular chemical measurement

Title:
Optochemical nanosensors for intracellular chemical measurement
Creator:
Kopelman, Raoul (Author)
Miller, Michael T. (Author)
Brasuel, Murphy (Author)
Clark, Heather (Author)
Hoyer, Marion (Author)
Philbert, Martin (Author)
Language:
English
Publisher:
SPIE
Copyright date:
1998
Type of resource:
Text
Genre:
Articles
Format:
electronic
Digital origin:
born digital
Abstract/Description:
The development of a submicron optical fiber "supertip" has provided advantages over previously produced submicron tips, such as facilitating insertion of these sensors into cells while minimizing damage to the cell membrane. Fiber optic ion correlation-based nanosensors for sodium, potassium and chloride employing these "supertips" have been applied to the monitoring of ion concentrations in single mouse oocytes. These sensors have also been used to monitor the effect of an ion channel-blocking agent. In order to address the challenge associated with single-cell simultaneous measurement of multiple analytes, the use of submicron optical fiber multiprobes has been explored.
Comments:
Originally published in SPIE Conference on Chemical, Biochemical, and Environmental Fiber Sensors X, Boston, MA, Nov. 1998, SPIE Vol. 3540, Proc. SPIE 3540, 198 (1999). DOI:10.1117/12.339797
Subjects and keywords:
Optodes
Optical detectors
Chemical detectors
optodes
chemical sensors
fiber optic
multiprobe
intracellular measurements
Bioimaging and biomedical optics
Permanent Link:
http://hdl.handle.net/2047/d20000325

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