Enumeration of sandy sediment bacteria

Title:
Enumeration of sandy sediment bacteria : search for optimal protocol
Creator:
Epstein, Slava S. (Author)
Rossel, Jeffrey (Author)
Language:
English
Publisher:
Inter-Research
Copyright date:
1995
Type of resource:
Text
Genre:
Articles
Format:
electronic
Digital origin:
born digital
Abstract/Description:
We examined and compared a variety of existing protocols for enumeration of bacteria from marine sandy sediments. The focus was on how to dislodge bacteria from sediment particles; a commercial blender, an ultrasonic cleaner, and an ultrasonic cell disrupter were tested. The ultrasonic cell disrupter was found to be the most efficient device for bacterial dislodgment. With a 5 mm microtip vibrating at 109 μm amplitude and 20 kHz, the optimal sonication time of small (≤ 0.5 cm3) samples was 180 s. Having identified the optimal dislodgment treatment, we went through other steps of sediment bacteria enumeration (use of surfactants, thoroughness of sample washing, bacterial staining and counterstaining, etc.), striving for additional ways of improvement. The result was a protocol that, in our study, gave bacterial counts superior to those obtained by any other protocol currently in use.
Comments:
Originally published in Marine Ecology Progress Series 117 (1995): 289-298. http://www.int-res.com/abstracts/meps/v117/
Subjects and keywords:
Marine bacteria - Counting
bacteria
benthos
enumeration
fluorescence
ultrasonic treatment
Bacteriology
Marine Biology

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